Journal: Scientific reports
Article Title: Serratia marcescens ATCC 274 increases production of the red pigment prodigiosin in response to Chi phage infection.
doi: 10.1038/s41598-024-68747-3
Figure Lengend Snippet: Figure 1. (A) Liquid cultures of S. marcescens wild type with and without the addition of 0.1 MOI χ phage. After 18 h of incubation at 25 °C with shaking, 10 ml of each culture was pipetted into one half of a split 10 cm petri dish. Plates were imaged with a Cytiva IQ800 instrument. Left, phage was added during exponential phase growth (OD600 = 0.5); Right, phage was added during stationary phase growth (OD600 = 2.0). (B) Pigmentation in S. marcescens wild type cultures as A535 quantified via spectrophotometer 18 h after addition of 0.1 MOI of χ phage to cultures in exponential phase (OD600 = 0.5) or stationary phase (OD600 = 2.0). Both sets are compared to a control sample without the addition of phage. Data points are mean values consisting of n = 3 replicates. Error bars indicate standard deviation. Student’s t-test was used to determine statistical significance. P values are represented by asterisks: *P < 0.05; ***P < 0.001.
Article Snippet: Strain, plasmid, or phage Parental strain/backbone Relevant characteristics Source Serratia marcescens ATCC 274 (WT) ATCC 274 Wild type Rasika Harshey ΔfliC ATCC 274 ΔfliC (flaF) This study ΔluxS ATCC 274 ΔluxS This study Δpig ATCC 274 ΔpigABCDEFGHIJKLMN This study ΔpigP ATCC 274 ΔSMATCC274_40570 This study pig-lacZ ATCC 274 ΔfliC; pigA::lacZ This study pigq ATCC 274 Ppig replaced with J23119 This study pKNG101 – MCS, strAB, sacB, mobRK2, oriV R6Kγ, traJK Sarah J Coulthurst pBS1315 pUC18 Cloning vector with J23119 promoter This study pBS1355 pKNG101 fliC (flaF) deletion construct This study pBS1359 pKNG101 pigq mutant construct This study pBS1361 pKNG101 pig operon deletion construct This study pBS1362 pKNG101 pig-lacZ fusion mutant construct This study DH5α-λpir Escherichia coli K-12 F – φ80lacZΔM15 Δ(lacZYA-argF)U169 recA1 endA1 hsdR17(rK–, mK+) phoA supE44 λ–thi-1 gyrA96 relA1 LAMpir Howard C. Berg SM10-λpir Escherichia coli K-12 thi thr leu tonA lacY supE recA::RP4-2-Tc::Mu KmLAMpir Clay Fuqua χ (Chi) – Wild type Saeed Tavazoie 10 Vol:. (1234567890) Scientific Reports | (2024) 14:17750 | https://doi.org/10.1038/s41598-024-68747-3 Although A535 values are often normalized to OD600 in other experiments described in the literature67, we elected not to normalize, due to the fact that phage-lysed cells and cell debris contribute to OD600.
Techniques: Incubation, Spectrophotometry, Control, Standard Deviation